Prof Sean Doyle
Biography
Oct 2022- Full Professor, Maynooth University.
2009-2022 Professor B, Maynooth University.
2001- 2008 Senior Lecturer, Maynooth University.
1997- 2001 Lecturer, Maynooth University.
1993-97 R&D Director, Biotrin, Dublin, Ireland.
1990-93 Senior Scientist, Wellcome Diagnostics, UK.
1989-90 Scientist/Manager, Unilever Research, UK.
1989 PhD Biochemistry, UCC, Ireland.
1985 BSc Biochemistry, UCC, Ireland.
Research Interests
To date, Doyle's molecular and applied proteomics work has resulted in 130 peer-reviewed publications to date, over 7500 citations (h-index: 47), patented fungal diagnostic technology (EP2709659, awarded 2018) and commercialised products. It is directed towards understanding microbial pathogenicity, antimicrobial resistance and identifying new biotechnological, therapeutic and diagnostic targets. See https://scholar.google.com/citations?user=DJhTVdEAAAAJ&hl=en
He was also instrumental as Lead Applicant in securing competitive SFI funding for Departmental quantitative mass spectrometry (MS) facilities (€566,540) which has enabled multiple new project awards within Biology at MU, MSc/PhD student projects - and is also available to internal & external users. All MS-related publications (129), equating to 1 per month since commissioning, to September 2023: https://www.maynoothuniversity.ie/system/temporary/filefield_paths/QP%20Publications%20Maynooth%202013-23_0.pdf
Doyle also secured competitive funding (€89,000) for Lightcycler 480 instrumentation which was offered, and loaned free-of-charge, to the National Virus Reference Laboratory (ultimately Enfer Scientific) from March - July 2020 to underpin essential and large-scale National molecular testing of patients for SARS-CoV-2, the causative agent of COVID-19.
The impact of this, and equivalent contributions by other Irish
Universities, on the establishment of control over what was an
exponentially evolving pandemic, cannot be underestimated.
Doyle has also successfully supervised 31 PhD and 10 MSc student projects to completion, and currently supervises 1 MSc student project. His group also has a number of academic and industrial collaborations and group members have been pro-active in the promotion of the public understanding of science through participation in such activities as the Maynooth University Chain Reaction Lecture Series and the National Science Week. Research work in the laboratory is funded by Science Foundation Ireland, Leeds-Beckett University and MU. Professor Doyle holds the distinction of Visiting Professor at Leeds Beckett University, UK.
Research area 1.
Aspergillus fumigatus: He studies the biology, diagnosis and molecular genetics of the human pathogenic fungus, Aspergillus fumigatus - a major cause of infection in immunocompromised individuals. His group has identified a number of potential virulence factors (proteins) in the organism, which may contribute both to its pathogenicity and emerging resistance to conventional chemotherapies. He was the first to identify and characterise a novel self-protection mechanism against gliotoxin in Aspergillus fumigatus. His group also identified a number of non-ribosomal peptide synthetases and glutathione s-transferases in A. fumigatus. Interestingly, non-ribosomal peptide synthetases in fungi represent a novel source of bioactive metabolites and his published work is at the cutting-edge of this internationally competitive area. The primary research methods involved in this work comprise gene deletion studies, proteomics, NMR and LC-MS.
Collaborators: Professor Gary Jones (Leeds-Beckett University, UK, Dr David Fitzpatrick, Dr Ozgur Bayram, Professor Hubertus Haas (Innsbruck, Austria), Professor Gustavo Goldman (Sao Paulo, Brazil), Dr Siobhan McClean (UCD) & Dr Maire Callaghan (ITT Dublin).
Research area 2
Immunodiagnostics: Using a well-established recombinant protein expression systems (E. coli and baculovirus) Doyle's group has expressed and continues to express A. fumigatus proteins in order to assess their diagnostic potential. In addition, he has developed novel immunoassays to detect fungal metabolites as disease indicators. In June 2020, he was awarded funding to develop COVID-19 ELISA and lateral flow-based immunodiagnostics. This work is in collaboration with Professor John P. Dalton, NUIG. Proteomic studies of a number of organisms, using Departmental protein MS facilities, are underway and peer-reviewed publications are emerging from these efforts. We are also engaged with improving the diagnosis of neonatal sepsis in Low-Middle Income Countries via SFI/Irish Aid-funded research.
Collaborators: Assoc Professor Dr Peter Waiswa, Makerere University School of Public Health & Global Health, Uganda; Dr Flavia Namiiro, Mulago General Hospital, Kampala, Uganda; Dr Nicola Mountford, School of Business, Maynooth University; Irish Neonatal Health Alliance; Accuplex Diagnostics Limited, Ireland.
Research area 3.
Armillaria mellea: In the past, this plant pathogenic fungus has
proven recalcitrant to detailed molecular analysis. However, Doyle with
Dr David Fitzpatrick has undertaken genome sequencing of this organism
and has established a robust proteomics pipeline to identify novel
biocatalysts (enzymes) with biotechnological, pharmaceutical and biofuel
applications. This work involves conventional individual protein mass
spectrometry and shotgun proteomics. The primary research methods
involved in this work comprise genome sequencing, bioinformatics,
proteomics, HPLC and small molecule LC-MS.
Collaborators: Dr David Fitzpatrick and Dr Rebecca Owens (Maynooth University).
Research equipment infrastructure available for Industry-Academia Collaborations:
The Department of Biology possesses a range of modern molecular and proteomic instrumentation which is used for both PhD and Post-Doctoral Research projects. This equipment, much of which has been funded by competitive awards from the HEA, HRB, SFI and Enterprise Ireland, is also available for use by companies on a commercial basis. For further details see:https://www.maynoothuniversity.ie/biology/instrumentation-facilities
Professor Sean Doyle- Applied and Discovery Research (2024)
1. Bioconjugation strategy development and process transfer
to manufacturing of:
(i) anti-human IgG-HRP conjugate (Hepatitis
C virus serodiagnostic systems; Wellcome Diagnostics, UK),
(ii) multiple IgG(anti-GST)-HRP conjugates
(Human, canine, porcine and murine GST detection systems; Biotrin) (Rees et
al., 1995),
(iii) biotinylated recombinant VP2 capsids
(FDA approved Parvovirus B19 IgM detection system, Biotrin) (Doyle et al., 2000),
(iv) chemical modification of haemoglobin
(Haptoglobin detection; Tridelta Development Limited; Eckersall et al., 1999) and
(v) Phosphorylcholine-BSA-HRP conjugation
(C-Reactive Protein detection; Tridelta Development Limited; Deegan et al.,
2003); First in class siderophore recombinant monoclonal antibody with diagnostic application- licensed (Moloney et al., 2021).
2. Bioconjugation strategies for immunization and chemoenzymatic
research applications:
(i) Fumagillin and helvolic acid
bioconjugation for immunogen synthesis (Fox et al., 2004),
(ii) synthesis of fluorescent and
biotinylated coenzyme A derivatives as substrates for novel 4’-phosphopantetheinyl
transferase activity determination by microwell assay (Stack et al., 2009),
(iii) fluorescent derivatization of
gliotoxin to enhance detection by RP-HPLC and MALDI-MS (Davis et al., 2011),
(iv) siderophore (fusarinine C (FsC) and triacetylfusarinine
C (TAFC)) conjugation to proteins as immunogens (EP patent awarded),
(v) fluorescent alkylation of antioxidant
ergothioneine (Gallagher et al., 2012),
(vi) siderophore (ornibactin) conjugation
to proteins as an immunogen (Whelan et al., 2015),
(vii) fluorescent derivatization of FsC
for fungal uptake studies (Moloney et al., 2016) and
(viii) simultaneous chemical acetylation
and activation of FsC for immunogen synthesis (Moloney et al., 2018).
3. Proteogenomic-mediated discovery of novel
biological systems:
Since 2004, Doyle has pioneered an integrated
proteogenomic approach to new systems discovery in fungi, which has led to the
first identification of:
(i) oxidoreductase
mediated self-resistance mechanisms against redox-active peptides,
(ii) negative
regulatory system for redox-active metabolite production,
(iii) new biosynthetic
functionality of detoxification enzymes,
(iv) integration of
primary and secondary metabolism via methylation systems,
(v) a novel metal
biochelator,
(vi) detailed
proteogenomic investigation of plant pathogen Armillaria mellea and
(vii) discovery
of supra-antioxidant, ergothioneine, presence and biosynthesis in Aspergillus fumigatus.
These proteogenomic strategies have involved fungal
growth under specific conditions, comparative quantitative proteomics to
identify altered proteins/pathways/systems, and subsequent gene deletion of
cognate genes to elucidate discrete
protein functionality.
Peer Reviewed Journal
| Year | Publication | |
|---|---|---|
| 2024 | Nowak, J., Ssanyu, J. N., Namiiro, F., Mountford, N., Parducci, A., Domijan, K., Daly, M., O’Brien, D., Barden, E., Walshe, K., Doyle S and Waiswa. P. (2024) 'Diagnosis of neonatal and adult sepsis using a Serum Amyloid A lateral flow test'. PLoS ONE, . [DOI] | |
| 2023 | Downes, S.G., Owens, R.A., Walshe, K., Fitzpatrick, D.A., Dorey, A.L., Jones, G.W. and Doyle, S. (2023) 'Gliotoxin-mediated bacterial growth inhibition is caused by specific metal ion depletion'. Scientific Reports, 13 (16156). [Link] [DOI] [Full-Text] | |
| 2023 | Corrales, J.L., McEvoy, A., Lalor, R., Cwiklinski, K., Doyle, S., Parkinson, M., Keane, O.M., Dalton, J.P. and Dorey, A. L. (2023) 'The use of cathepsin L1 (FhCL1) serological ELISA in sentinel screening for liver fluke on sheep farms'. Veterinary Parasitology, Regional Studies And Reports, 100924 . [Link] [DOI] | |
| 2023 | Katie Dunne, Emma Reece, Siobhán McClean, Sean Doyle, Thomas Rogers, Philip Murphy, Julie Renwick (2023) 'Aspergillus fumigatus Supernatants Disrupt Bronchial Epithelial Monolayers: Potential Role For Enhanced Invasion In Cystic Fibrosis'. Journal Of Fungi, 9 (4). [Link] | |
| 2023 | Traynor, A.M., Sarikaya-Bayram, Ö., Bayram, Ö., Calera J.A., and Doyle, S. (2023) 'Proteomic dissection of role of GliZ in gliotoxin biosynthesis in Aspergillus fumigatus'. Fungal Genetics and Biology, 166 (103795). [Link] [DOI] [Full-Text] | |
| 2023 | Borges, BM; Ramos, RBC; Preite, NW; Kaminski, VL; Alves de Castro, P; Camacho, M; Maximo, MF; Fill, TP; Calich, VLG; Traynor, AM; Sarikaya-Bayram, Ö; Doyle, S; Bayram, Ö; de Campos, CBL; Zelanis, A; Goldman, GH; Loures, FV (2023) 'Transcriptional profiling of a fungal granuloma reveals a low metabolic activity of Paracoccidioides brasiliensis yeasts and an actively regulated host immune response'. Frontiers in Cellular and Infection Microbiology, 13 . [DOI] [Full-Text] | |
| 2023 | Le Berre, M., Paulovčáková, T., De Marco Verissimo, C., Doyle, S., Dalton, J.P., Masterson, C., Ribes Martinez, E., Walsh, L., Gormley, C., Laffey, J.G., McNicholas, B., Simpkin, A. and Kilcoyne M. (2023) 'A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time'. PLoS ONE, . [Full-Text] | |
| 2023 | Downes, S.G., Doyle, S., Jones G.W. and Owens, R.A. (2023) 'Gliotoxin and related metabolites as zinc chelators: implications and exploitation to overcome antimicrobial resistance'. Essays in Biochemistry, . [Link] [DOI] | |
| 2023 | Smith H.; Doyle S.; Murphy R. (2023) 'Target directed identification of natural bioactive compounds from filamentous fungi'. Food Chemistry, 405 . [DOI] [Full-Text] | |
| 2022 | Doyle S.; Cuskelly D.D.; Conlon N.; Fitzpatrick D.A.; Gilmartin C.B.; Dix S.H.; Jones G.W. (2022) 'A Single Aspergillus fumigatus Gene Enables Ergothioneine Biosynthesis and Secretion by Saccharomyces cerevisiae'. International Journal of Molecular Sciences, 23 (18). [DOI] [Full-Text] | |
| 2022 | Conlon N., Murphy, R.A., Corrigan A., Doyle S., Owens R.A. and Fagan S. (2022) 'Quantitative Proteomic Analysis Reveals Yeast Cell Wall Products Influence the Serum Proteome Composition of Broiler Chickens'. International Journal of Molecular Sciences, 23 (19):1-27. [Link] [DOI] | |
| 2022 | Verissimo C.D.M.; López-Corrales J.; Dorey A.L.; Cwiklinski K.; Lalor R.; Calvani N.E.D.; Jewhurst H.L.; Flaus A.; Doyle S.; Dalton J.P. (2022) 'Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-Like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system'. Epidemiology and Infection, . [DOI] [Full-Text] | |
| 2022 | Anna Rawlings, Eoin O'Connor, Suzy Moody, Ed Dudley, Lynne Boddy, Mike Fowler, David A. Fitzpatrick, Sean Doyle, Daniel Eastwood. (2022) 'Metabolic responses of two pioneer wood decay fungi to diurnally cycling temperature'. JOURNAL OF ECOLOGY, 110 (1):68-79. [Link] [DOI] [Full-Text] | |
| 2021 | Xu, LN; Zhang, H; Cuskelly, DD; Doyle, S; Perrett, S; Jones, GW (2021) 'Mutational analysis of the Hsp70 substrate-binding domain: Correlating molecular-level changes with in vivo function'. Molecular Microbiology, . [DOI] [Full-Text] | |
| 2021 | O'Connor E.; Doyle S.; Amini A.; Grogan H.; Fitzpatrick D.A. (2021) 'Transmission of mushroom virus X and the impact of virus infection on the transcriptomes and proteomes of different strains of Agaricus bisporus'. Fungal Biology, . [DOI] [Full-Text] | |
| 2021 | Verissimo C.D.M.; O'brien C.; López Corrales J.; Dorey A.; Cwiklinski K.; Lalor R.; Doyle J.M.; Field S.; Masterson C.; Ribes Martinez E.; Hughes G.; Bergin C.; Walshe K.; Mcnicholas B.; Laffey J.G.; Dalton J.P.; Kerr C.; Doyle S. (2021) 'Improved diagnosis of SARS-CoV-2 by using Nucleoprotein and Spike protein fragment 2 in quantitative dual ELISA tests'. Epidemiology and Infection, . [DOI] [Full-Text] | |
| 2021 | Traynor, A.M., Owens, R.A., Coughlin, C.M., Holton, M.C., Jones, G.W., Calera, J.A. and Doyle. S. (2021) 'At the metal-metabolite interface in Aspergillus fumigatus: towards untangling the intersecting roles of zinc and gliotoxin'. MICROBIOL-SGM, 167 (11):1-15. [Link] [DOI] [Full-Text] | |
| 2021 | Moloney N.M.; Larkin A.; Xu L.; Fitzpatrick D.A.; Crean H.L.; Walshe K.; Haas H.; Decristoforo C.; Doyle S. (2021) 'Generation and characterisation of a semi-synthetic siderophore-immunogen conjugate and a derivative recombinant triacetylfusarinine C–specific monoclonal antibody with fungal diagnostic application'. Analytical Biochemistry, 632 . [DOI] [Full-Text] | |
| 2021 | Owens, R.A. and Doyle, S. (2021) 'Effects of antifungal agents on the fungal proteome: Informing on mechanisms of sensitivity and resistance'. Expert Rev Proteomic, . [Link] [DOI] | |
| 2020 | Scott, J; Sueiro-Olivares, M; Thornton, BP; Owens, RA; Muhamadali, H; Fortune-Grant, R; Thomson, D; Thomas, R; Hollywood, K; Doyle, S; Goodacre, R; Tabernero, L; Bignell, E; Amich, J (2020) 'Targeting Methionine Synthase in a Fungal Pathogen Causes a Metabolic Imbalance That Impacts Cell Energetics, Growth, and Virulence'. mBio, 11 . [DOI] [Full-Text] | |
| 2020 | O'Connor E.; Owens R.A.; Doyle S.; Amini A.; Grogan H.; Fitzpatrick D.A. (2020) 'Proteomic investigation of interhyphal interactions between strains of Agaricus bisporus'. Fungal Biology, 124 (6):579-591. [DOI] [Full-Text] | |
| 2020 | Ries LNA; Pardeshi L; Dong Z; Tan K; Steenwyk JL; Colabardini AC; Ferreira Filho JA; de Castro PA; Silva LP; Preite NW; Almeida F; de Assis LJ; Dos Santos RAC; Bowyer P; Bromley M; Owens RA; Doyle S; Demasi M; Hernández DCR; Netto LES; Pupo MT; Rokas A; Loures FV; Wong KH; Goldman GH; (2020) 'The Aspergillus fumigatus transcription factor RglT is important for gliotoxin biosynthesis and self-protection, and virulence'. PLoS Pathogens, 16 (7). [DOI] [Full-Text] | |
| 2019 | Waldron R; McGowan J; Gordon N; Mitchell EB; Fitzpatrick DA; Doyle S; (2019) 'Characterisation of three novel β-1,3 glucanases from the medically important house dust mite Dermatophagoides pteronyssinus (airmid)'. Insect Biochemistry and Molecular Biology, 115 . [DOI] [Full-Text] | |
| 2019 | Traynor, A.M., Sheridan, K.J., Jones, G.W., Calera, J.A. and Doyle S. (2019) 'Involvement of Sulfur in the Biosynthesis of Essential Metabolites in Pathogenic Fungi of Animals, particularly Aspergillus spp.: Molecular and Therapeutic Implications'. Frontiers in Microbiology, 10 (2859):1-16. [Link] [DOI] [Full-Text] | |
| 2019 | Xu L.; Nitika; Hasin N.; Cuskelly D.; Wolfgeher D.; Doyle S.; Moynagh P.; Perrett S.; Jones G.; Truman A. (2019) 'Rapid deacetylation of yeast Hsp70 mediates the cellular response to heat stress'. Scientific Reports, 9 (1). [DOI] [Full-Text] | |
| 2019 | Sarikaya Bayram Ö; Dettmann A; Karahoda B; Moloney NM; Ormsby T; McGowan J; Cea-Sánchez S; Miralles-Durán A; Brancini GTP; Luque EM; Fitzpatrick DA; Cánovas D; Corrochano LM; Doyle S; Selker EU; Seiler S; Bayram Ö (2019) 'Control of development, secondary metabolism and light-dependent carotenoid biosynthesis by the velvet complex of Neurospora crassa'. GENETICS, . https://doi.org/10.1534/genetics.119.302277 [Full-Text] | |
| 2018 | Saleh A.; Jones G.; Tinley F.; Delaney S.; Alabbadi S.; Fenlon K.; Doyle S.; Owens R. (2018) 'Systems impact of zinc chelation by the epipolythiodioxopiperazine dithiol gliotoxin in: Aspergillus fumigatus: A new direction in natural product functionality'. Metallomics, 10 (6):854-866. [DOI] [Full-Text] | |
| 2018 | Reece E.; Doyle S.; Greally P.; Renwick J.; McClean S. (2018) 'Aspergillus fumigatus inhibits Pseudomonas aeruginosa in co-culture: Implications of a mutually antagonistic relationship on virulence and inflammation in the CF airway'. Frontiers in Microbiology, 9 (JUN). [DOI] [Full-Text] | |
| 2018 | Cullen L.; O’Connor A.; McCormack S.; Owens R.; Holt G.; Collins C.; Callaghan M.; Doyle S.; Smith D.; Schaffer K.; Fitzpatrick D.; McClean S. (2018) 'The involvement of the low-oxygen-activated locus of Burkholderia cenocepacia in adaptation during cystic fibrosis infection'. Scientific Reports, 8 (1). [DOI] [Full-Text] | |
| 2018 | Doyle S., Jones G.W and Dolan S.K. (2018) 'Dysregulated Gliotoxin Biosynthesis Attenuates the Production of Unrelated Biosynthetic Gene Cluster-Encoded Metabolites in Aspergillus fumigatus'. Fungal Biology, 122 (4):214-221. [Link] [DOI] [Full-Text] | |
| 2017 | Delgado J.; Owens R.; Doyle S.; Núñez F.; Asensio M. (2017) 'Quantitative proteomics reveals new insights into calcium-mediated resistance mechanisms in Aspergillus flavus against the antifungal protein PgAFP in cheese'. Food Microbiology, 66 :1-10. [DOI] [Full-Text] | |
| 2017 | Mulvihill E.; Moloney N.; Owens R.; Dolan S.; Russell L.; Doyle S. (2017) 'Functional investigation of iron-responsive microsomal proteins, including MirC, in Aspergillus fumigatus'. Frontiers in Microbiology, 8 (MAR). [DOI] [Full-Text] | |
| 2017 | Sipos G; Prasanna AN; Walter MC; O'Connor E; Bálint B; Krizsán K; Kiss B; Hess J; Varga T; Slot J; Riley R; Bóka B; Rigling D; Barry K; Lee J; Mihaltcheva S; LaButti K; Lipzen A; Waldron R; Moloney NM; Sperisen C; Kredics L; Vágvölgyi C; Patrignani A; Fitzpatrick D; Nagy I; Doyle S; Anderson JB; Grigoriev IV; Güldener U; Münsterkötter M; Nagy LG; (2017) 'Genome expansion and lineage-specific genetic innovations in the forest pathogenic fungi Armillaria'. Nature Ecology & Evolution, . [DOI] [Full-Text] | |
| 2017 | Waldron R; McGowan J; Gordon N; McCarthy C; Mitchell EB; Doyle S; Fitzpatrick DA; (2017) 'Draft Genome Sequence of Dermatophagoides pteronyssinus, the European House Dust Mite'. Genome Announcements, 5 (32). [DOI] [Full-Text] | |
| 2017 | Manfiolli AO; de Castro PA; Dos Reis TF; Dolan S; Doyle S; Jones G; Riaño Pachón DM; Ulaş M; Noble LM; Mattern DJ; Brakhage AA; Valiante V; Silva-Rocha R; Bayram O; Goldman GH; (2017) 'Aspergillus fumigatus protein phosphatase PpzA is involved in iron assimilation, secondary metabolite production, and virulence'. Cellular Microbiology, . [DOI] [Full-Text] | |
| 2017 | Dolan S.K, Bock, T., Hering, V., Owens, R.A., Jones, G.W., Blankenfeldt W., Doyle S. (2017) 'Structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in Aspergillus fumigatus'. Open Biology, 7 . [Link] [DOI] [Full-Text] | |
| 2016 | Delgado, J; Owens, RA; Doyle, S; Asensio, MA; Núñez, F (2016) 'Increased chitin biosynthesis contributes to the resistance of Penicillium polonicum against the antifungal protein PgAFP'. Applied Microbiology and Biotechnology, 100 (1):371-383. [DOI] [Full-Text] | |
| 2016 | Delgado J; Owens RA; Doyle S; Asensio MA; Núñez F; (2016) 'Antifungal proteins from moulds: analytical tools and potential application to dry-ripened foods'. Applied Microbiology and Biotechnology, 100 (16):6991-6700. [DOI] [Full-Text] | |
| 2016 | de Castro PA; Dos Reis TF; Dolan SK; Manfiolli AO; Brown NA; Jones GW; Doyle S; Riaño-Pachón DM; Squina FM; Caldana C; Singh A; Del Poeta M; Hagiwara D; Silva-Rocha R; Goldman GH; (2016) 'The Aspergillus fumigatus SchA(SCH9) kinase modulates SakA(HOG1) MAP kinase activity and it is essential for virulence'. Molecular Microbiology, . [DOI] [Full-Text] | |
| 2016 | Sheridan KJ; Lechner BE; Keeffe GO; Keller MA; Werner ER; Lindner H; Jones GW; Haas H; Doyle S; (2016) 'Ergothioneine Biosynthesis and Functionality in the Opportunistic Fungal Pathogen, Aspergillus fumigatus'. Scientific Reports, 6 . [DOI] [Full-Text] | |
| 2016 | McClean S; Healy ME; Collins C; Carberry S; O'Shaughnessy L; Dennehy R; Adams Á; Kennelly H; Corbett JM; Carty F; Cahill LA; Callaghan M; English K; Mahon BP; Doyle S; Shinoy M; (2016) 'Linocin and OmpW Are Involved in Attachment of the Cystic Fibrosis-Associated Pathogen Burkholderia cepacia Complex to Lung Epithelial Cells and Protect Mice against Infection'. Infection and Immunity, 84 (5). [DOI] [Full-Text] | |
| 2016 | Amich, J., Dmig M., O'Keeffe, G., Binder, J., Doyle, S., Beilhack, A. and Krappmann, S. (2016) 'Exploring sulfur assimilation of Aspergillus fumigatus reveals biosynthesis of sulfur-containing amino acids as a virulence determinant'. Infection and Immunity, Accepted . [DOI] [Full-Text] | |
| 2016 | Moloney, NM*; Owens, RA*; Meleady, P; Henry, M; Dolan, SK; Mulvihill, E; Clynes, M; Doyle, S (2016) 'The iron-responsive microsomal proteome of Aspergillus fumigatus'. JOURNAL OF PROTEOMICS, 136 :99-111. [DOI] [Full-Text] | |
| 2016 | Elizabeth B. Smith, Stephen K. Dolan, David A. Fitzpatrick, Sean Doyle and Gary W. Jones (2016) 'Towards understanding the gliotoxin detoxification mechanism: in vivo thiomethylation protects yeast from gliotoxin cytotoxicity'. Microbial Cell, 3 (3):120-125. [Link] [DOI] [Full-Text] | |
| 2016 | Moloney NM; Owens RA; Doyle S; (2016) 'Proteomic analysis of Aspergillus fumigatus - clinical implications'. Expert Review of Proteomics, . [DOI] | |
| 2016 | Manzanares-Miralles, L; Sarikaya-Bayram, O; Smith, EB; Dolan, SK; Bayram, O; Jones, GW; Doyle, S (2016) 'Quantitative proteomics reveals the mechanism and consequence of gliotoxin-mediated dysregulation of the methionine cycle in Aspergillus niger'. JOURNAL OF PROTEOMICS, 131 :149-162. [DOI] [Full-Text] | |
| 2015 | Delgado, J; Owens, RA; Doyle, S; Asensio, MA; Nunez, F (2015) 'Impact of the antifungal protein PgAFP from Penicillium chrysogenum on the protein profile in Aspergillus flavus'. Applied Microbiology and Biotechnology, 99 :8701-8715. [DOI] [Full-Text] | |
| 2015 | Smith, H; Doyle, S; Murphy, R (2015) 'Filamentous fungi as a source of natural antioxidants'. Food Chemistry, 185 :389-397. [Full-Text] | |
| 2015 | Sheridan, KJ; Dolan, SK; Doyle, S (2015) 'Endogenous cross-talk of fungal metabolites'. Frontiers in Microbiology, 5 . [DOI] [Full-Text] | |
| 2015 | Culleton, BA; Lall, P; Kinsella, GK; Doyle, S; McCaffrey, J; Fitzpatrick, DA; Burnell, AM (2015) 'A role for the Parkinson's disease protein DJ-1 as a chaperone and antioxidant in the anhydrobiotic nematode Panagrolaimus superbus'. Cell Stress and Chaperones, 20 :121-137. [DOI] [Full-Text] | |
| 2015 | Winkelstroter, LK; Dolan, SK; dos Reis, TF; Bom, VLP; de Castro, PA; Hagiwara, D; Alowni, R; Jones, GW; Doyle, S; Brown, NA; Goldman, GH (2015) 'Systematic Global Analysis of Genes Encoding Protein Phosphatases in Aspergillus fumigatus'. G3 (Bethesda, Md.), 5 :1525-1539. [DOI] [Full-Text] | |
| 2015 | Owens, RA; O'Keeffe, G; Smith, EB; Dolan, SK; Hammel, S; Sheridan, KJ; Fitzpatrick, DA; Keane, TM; Jones, GW; Doyle, S (2015) 'Interplay between Gliotoxin Resistance, Secretion, and the Methyl/Methionine Cycle in Aspergillus fumigatus'. Eukaryotic Cell, 14 :941-957. [DOI] [Full-Text] | |
| 2015 | Dolan, S.K., O’Keeffe, G., Jones, G.W. and Doyle S. (2015) 'Resistance is not futile: gliotoxin biosynthesis, functionality and utility'. Trends in Microbiology, 23 :419-428. [DOI] [Full-Text] | |
| 2015 | Fagan S.; Owens R.; Ward P.; Connolly C.; Doyle S.; Murphy R. (2015) 'Biochemical Comparison of Commercial Selenium Yeast Preparations'. Biological Trace Element Research, . [DOI] [Full-Text] | |
| 2014 | O'Loughlin, A; McGee, M; Doyle, S; Earley, B (2014) 'Biomarker responses to weaning stress in beef calves'. Research in Veterinary Science, 97 :458-463. [DOI] [Full-Text] | |
| 2014 | Dolan, SK; Owens, RA; O'Keeffe, G; Hammel, S; Fitzpatrick, DA; Jones, GW; Doyle, S (2014) 'Regulation of Nonribosomal Peptide Synthesis: bis-Thiomethylation Attenuates Gliotoxin Biosynthesis in Aspergillus fumigatus'. Chemistry and Biology, 21 :999-1012. [DOI] [Full-Text] | |
| 2014 | Jones, GW; Doyle, S; Fitzpatrick, DA (2014) 'The evolutionary history of the genes involved in the biosynthesis of the antioxidant ergothioneine'. Gene, 549 :161-170. [DOI] [Full-Text] | |
| 2014 | Owens, RA; Hammel, S; Sheridan, KJ; Jones, GW; Doyle, S (2014) 'A Proteomic Approach to Investigating Gene Cluster Expression and Secondary Metabolite Functionality in Aspergillus fumigatus'. PLoS ONE, 9 . [DOI] [Full-Text] | |
| 2014 | O'Keeffe, G; Hammel, S; Owens, RA; Keane, TM; Fitzpatrick, DA; Jones, GW; Doyle, S (2014) 'RNA-seq reveals the pan-transcriptomic impact of attenuating the gliotoxin self-protection mechanism in Aspergillus fumigatus'. BMC Genomics, 15 . [DOI] [Full-Text] | |
| 2014 | Johnson G., Ferrini A., Dolan, S.K., Nolan T., Agrawal S., Doyle S. and Bustin S. (2014) 'Biomarkers for Invasive Aspergillosis: the challenges continue'. Biomarkers in Medicine, 8 :429-451. [DOI] [Full-Text] | |
| 2013 | Collins, C; Keane, TM; Turner, DJ; O'Keeffe, G; Fitzpatrick, DA; Doyle, S (2013) 'Genomic and Proteomic Dissection of the Ubiquitous Plant Pathogen, Armillaria mellea: Toward a New Infection Model System'. Journal of Proteome Research, 12 :2552-2570. [DOI] [Full-Text] | |
| 2013 | Keady, SM; Kenny, DA; Ohlendieck, K; Doyle, S; Keane, MG; Waters, SM (2013) 'Proteomic profiling of bovine M-longissimus lumborum from Crossbred Aberdeen Angus and Belgian Blue sired steers varying in genetic merit for carcass weight'. Journal of Animal Science, 91 :654-665. [DOI] [Full-Text] | |
| 2013 | O'Keeffe, G; Jochl, C; Kavanagh, K; Doyle, S (2013) 'Extensive proteomic remodeling is induced by eukaryotic translation elongation factor 1B gamma deletion in Aspergillus fumigatus'. Protein Science, 22 :1612-1622. [DOI] [Full-Text] | |
| 2013 | Shinoy, M; Dennehy, R; Coleman, L; Carberry, S; Schaffer, K; Callaghan, M; Doyle, S; McClean, S (2013) 'Immunoproteomic Analysis of Proteins Expressed by Two Related Pathogens, Burkholderia multivorans and Burkholderia cenocepacia, during Human Infection'. PLoS ONE, 8 . [DOI] [Full-Text] | |
| 2012 | O'Hanlon, KA; Margison, GP; Hatch, A; Fitzpatrick, DA; Owens, RA; Doyle, S; Jones, GW (2012) 'Molecular characterization of an adaptive response to alkylating agents in the opportunistic pathogen Aspergillus fumigatus'. Nucleic Acids Research, 40 :7806-7820. [DOI] [Full-Text] | |
| 2012 | O'Loughlin, A; Lynn, DJ; Mcgee, M; Doyle, S; McCabe, M; Earley, B (2012) 'Transcriptomic analysis of the stress response to weaning at housing in bovine leukocytes using RNA-seq technology'. BMC Genomics, 13 . [DOI] [Full-Text] | |
| 2012 | Lynch, EM; McGee, M; Doyle, S; Earley, B (2012) 'Effect of pre-weaning concentrate supplementation on peripheral distribution of leukocytes, functional activity of neutrophils, acute phase protein and behavioural responses of abruptly weaned and housed beef calves'. BMC Veterinary Research, 8 . [DOI] [Full-Text] | |
| 2012 | Gallagher, L; Owens, RA; Dolan, SK; O'Keeffe, G; Schrettl, M; Kavanagh, K; Jones, GW; Doyle, S (2012) 'The Aspergillus fumigatus Protein GliK Protects against Oxidative Stress and Is Essential for Gliotoxin Biosynthesis'. Eukaryotic Cell, 11 :1226-1238. [DOI] [Full-Text] | |
| 2012 | O'Hanlon, KA; Gallagher, L; Schrettl, M; Jochl, C; Kavanagh, K; Larsen, TO; Doyle, S (2012) 'Nonribosomal Peptide Synthetase Genes pesL and pes1 Are Essential for Fumigaclavine C Production in Aspergillus fumigatus'. Applied And Environmental Microbiology, 78 :3166-3176. [DOI] [Full-Text] | |
| 2012 | Carberry, S; Molloy, E; Hammel, S; O'Keeffe, G; Jones, GW; Kavanagh, K; Doyle, S (2012) 'Gliotoxin effects on fungal growth: Mechanisms and exploitation'. Fungal Genetics and Biology, 49 :302-312. [DOI] [Full-Text] | |
| 2012 | Marques, MPC; Walshe, K; Doyle, S; Fernandes, P; de Carvalho, CCCR (2012) 'Anchoring high-throughput screening methods to scale-up bioproduction of siderophores'. Process Biochemistry, 47 :416-421. [DOI] [Full-Text] | |
| 2011 | O'Shaughnessy, L; Carr, M; Crowley, B; Carberry, S; Doyle, S (2011) 'Recombinant expression and immunological characterisation of proteins derived from human metapneumovirus'. Journal of Clinical Virology, 52 :236-243. [DOI] [Full-Text] | |
| 2011 | O'Hanlon, KA; Cairns, T; Stack, D; Schrettl, M; Bignell, EM; Kavanagh, K; Miggin, SM; O'Keeffe, G; Larsen, TO; Doyle, S (2011) 'Targeted Disruption of Nonribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus'. Infection and Immunity, 79 :3978-3992. [DOI] [Full-Text] | |
| 2011 | Lynch, EM; McGee, M; Doyle, S; Earley, B (2011) 'Effect of post-weaning management practices on physiological and immunological responses of weaned beef calves'. Irish Journal Of Agricultural And Food Research, 50 :161-174. [Full-Text] | |
| 2011 | Davis, C; Gordon, N; Murphy, S; Singh, I; Kavanagh, K; Carberry, S; Doyle, S (2011) 'Single-pot derivatisation strategy for enhanced gliotoxin detection by HPLC and MALDI-ToF mass spectrometry'. Analytical and Bioanalytical Chemistry, 401 :2519-2529. [DOI] [Full-Text] | |
| 2011 | O'Shaughnessy L.; Doyle S. (2011) 'Purification of proteins from baculovirus-infected insect cells'. Methods in molecular biology (Clifton, N.J.), 681 :295-309. | |
| 2011 | Doyle S. (2011) 'The detection of parvoviruses'. Methods in molecular biology (Clifton, N.J.), 665 :213-231. [Full-Text] | |
| 2011 | O'Loughlin, A; McGee, M; Waters, SM; Doyle, S; Earley, B (2011) 'Examination of the bovine leukocyte environment using immunogenetic biomarkers to assess immunocompetence following exposure to weaning stress'. BMC Veterinary Research, 7 . [DOI] [Full-Text] | |
| 2011 | Davis, C; Carberry, S; Schrettl, M; Singh, I; Stephens, JC; Barry, SM; Kavanagh, K; Challis, GL; Brougham, D; Doyle, S (2011) 'The Role of Glutathione S-Transferase GliG in Gliotoxin Biosynthesis in Aspergillus fumigatus'. Chemistry and Biology, 18 :542-552. [DOI] [Full-Text] | |
| 2010 | Schrettl, M; Carberry, S; Kavanagh, K; Haas, H; Jones, GW; O'Brien, J; Nolan, A; Stephens, J; Fenelon, O; Doyle, S (2010) 'Self-Protection against Gliotoxin-A Component of the Gliotoxin Biosynthetic Cluster, GliT, Completely Protects Aspergillus fumigatus Against Exogenous Gliotoxin'. PLoS Pathogens, 6 . [DOI] [Full-Text] | |
| 2010 | Lynch E.; Earley B.; McGee M.; Doyle S. (2010) 'Characterisation of physiological and immunological responses in beef cows to abrupt weaning and subsequent housing'. BMC Veterinary Research, 6 . [DOI] [Full-Text] | |
| 2010 | Lynch, EM; Earley, B; McGee, M; Doyle, S (2010) 'Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves'. BMC Veterinary Research, 6 . [DOI] [Full-Text] | |
| 2010 | Lynch, E.M., Earley, B. McGee, M., Byrne, W. and Doyle, S. (2010) 'Characterisation of physiological, haematological and immunological responses in beef cows to abrupt weaning and subsequent housing'. BMC Veterinary Research, 6 . [DOI] [Full-Text] | |
| 2009 | Gaffney, M; Doyle, S; Murphy, R (2009) 'Optimization of Xylanase Production by Thermomyces lanuginosus in Solid State Fermentation'. Bioscience, Biotechnology and Biochemistry, 73 :2640-2644. [DOI] [Full-Text] | |
| 2009 | Stack, D; Frizzell, A; Tomkins, K; Doyle, S (2009) 'Solid Phase 4 '-Phosphopantetheinylation: Fungal Thiolation Domains are Targets for Chemoenzymatic Modification'. Bioconjugate Chemistry, 20 :1514-1522. [DOI] [Full-Text] | |
| 2009 | Gaffney, M., Carberry, S., Doyle S and Murphy, R. (2009) 'Purification and characterisation of a xylanase from Thermomyces lanuginosus and subsequent expression by Pichia pastoris'. Enzyme and Microbial Technology, 45 :348-354. [Full-Text] | |
| 2007 | Carberry, S. and S. Doyle (2007) 'Proteomic studies in biomedically and industrially relevant fungi'. Cytotechnology, 53 :95-100. [Full-Text] | |
| 2007 | Corcoran, A., S. Kerr, M. Koppelman and S. Doyle (2007) 'Improved detection of acute parvovirus B19 infection by IgM EIA in combination with a novel antigen EIA'. Vox Sanguinis, 93 :216-222. [Full-Text] | |
| 2007 | Stack D., Neville C.M. and Doyle S (2007) 'Nonribosomal Peptide Synthesis in the Human Pathogen Aspergillus fumigatus and other Fungi'. 153 :1297-1306. [Full-Text] | |
| 2007 | Vickers, I; Reeves, EP; Kavanagh, KA; Doyle, S (2007) 'Isolation, activity and immunological characterisation of a secreted aspartic protease, CtsD, from Aspergillus fumigatus'. Protein Expression and Purification, 53 :216-224. [DOI] [Full-Text] | |
| 2006 | Reeves, EP; Reiber, K; Neville, C; Scheibner, O; Kavanagh, K; Doyle, S (2006) 'A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus'. FEBS Journal, 273 :3038-3053. [DOI] [Full-Text] | |
| 2006 | Corcoran, A; Cromley, B; Dewhurst, C; Pizer, BL; Doyle, S (2006) 'Establishment of functional B cell memory against parvovirus B19 capsid proteins may be associated with resolution of persistent infection'. Journal of Medical Virology, 78 :125-128. [DOI] [Full-Text] | |
| 2006 | Doyle, S; Corcoran, A (2006) 'The immune response to parvovirus B19 exposure in previously seronegative and seropositive individuals'. Journal of Infectious Diseases, 194 :154-158. [Full-Text] | |
| 2006 | Carberry, S; Neville, CM; Kavanagh, KA; Doyle, S (2006) 'Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: Identification and characterisation of an elongation factor 1B protein with glutathione transferase activity'. Biochemical and Biophysical Research Communications, 341 :1096-1104. [DOI] [Full-Text] | |
| 2005 | Neville, C; Murphy, A; Kavanagh, K; Doyle, S (2005) 'A 4 '-phosphopantetheinyl transferase mediates non-ribosomal peptide synthetase activation in Aspergillus fumigatus'. ChemBioChem, 6 :679-685. [DOI] [Full-Text] | |
| 2005 | Burns, C; Geraghty, R; Neville, C; Murphy, A; Kavanagh, K; Doyle, S (2005) 'Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus'. Fungal Genetics and Biology, 42 :319-327. [DOI] [Full-Text] | |
| 2005 | Waters, SM; Doyle, S; Murphy, RA; Power, RFG (2005) 'Development of solution phase hybridisation PCR-ELISA for the detection and quantification of Enterococcus faecalis and Pediococcus pentosaceus in Nurmi-type cultures'. Journal of Microbiological Methods, 63 :264-275. [DOI] [Full-Text] | |
| 2005 | Wallace K, Elrick L, Leel V, Durward E, Doyle S, Kavanagh K, Porter A, Wright MC. (2005) 'Targeting therapies for the treatment of liver fibrosis - Chemical conjugation of gliotoxin to a human small chain antibody selected for its affinity to a surface antigen on human hepatic stellate cells'. Journal of Hepatology, 42 :601A-602A. | |
| 2005 | Reiber K.; Reeves E.; Neville C.; Winkler R.; Gebhardt P.; Kavanagh K.; Doyle S. (2005) 'The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron'. Fems Microbiology Letters, 248 (1):83-91. [DOI] [Full-Text] | |
| 2005 | Corcoran, A. and Doyle, S. (2005) 'Evidence of serological cross-reactivity between genotype 1 and genotype 3 erythrovirus infections'. 79 :5238-5239. [Full-Text] | |
| 2004 | Collins, PR; Stack, CM; O'Neill, SM; Doyle, S; Ryan, T; Brennan, GP; Mousley, A; Stewart, M; Maule, AG; Dalton, JP; Donnelly, S (2004) 'Cathepsin L1, the major protease involved in liver fluke (Fasciola hepatica) virulence - Propeptide cleavage sites and autoactivation of the zymogen secreted from gastrodermal cells'. Journal of Biological Chemistry, 279 :17038-17046. [DOI] [Full-Text] | |
| 2004 | Corcoran, A., Mahon, B.P. and Doyle, S. (2004) 'B cell memory is directed towards conformational epitopes of parvovirus B19 capsid proteins and the VP1-unique region'. Journal of Infectious Diseases, 189 :1873-1880. [Full-Text] | |
| 2004 | Corcoran, A. and Doyle, S. (2004) 'Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19'. Journal of Medical Microbiology, 53 :459-475. [Full-Text] | |
| 2004 | McCarthy E., Stack, C., Donnelly, S.M., Doyle, S., Mann, V.H., Brindley, P.J., Brennan, G., Stewart, M., Maule, A.G., and Dalton, J.P. (2004) 'Leucine aminopeptidase of the human blood fluke, Schistosoma mansoni'. International Journal For Parasitology, 34 :703-714. [Full-Text] | |
| 2004 | Fox, M; Gray, G; Kavanagh, K; Lewis, C; Doyle, S (2004) 'Detection of Aspergillus fumigatus mycotoxins: immunogen synthesis and immunoassay development'. Journal of Microbiological Methods, 56 :221-230. [DOI] [Full-Text] | |
| 2004 | McCarthy, E; Stack, C; Donnelly, SM; Doyle, S; Mann, VH; Brindley, PJ; Stewart, M; Day, TA; Maule, AG; Dalton, JP (2004) 'Leucine aminopeptidase of the human blood flukes, Schistosoma mansoni and Schistosoma japonicum'. International Journal For Parasitology, 34 :703-714. [DOI] [Full-Text] | |
| 2004 | Corcoran, A; Mahon, BP; Doyle, S (2004) 'B cell memory is directed toward conformational epitopes of parvovirus B19 capsid proteins and the unique region of VP1'. Journal of Infectious Diseases, 189 :1873-1880. [Full-Text] | |
| 2004 | Reeves, EP; Messina, CGM; Doyle, S; Kavanagh, K (2004) 'Correlation between gliotoxin production and virulence of Aspergillus fumigatus in Galleria mellonella'. Mycopathologia, 158 :73-79. [Full-Text] | |
| 2003 | O'Keeffe, J; Doyle, S; Kavanagh, K (2003) 'Exposure of the yeast Candida albicans to the anti-neoplastic agent adriamycin increases the tolerance to amphotericin B'. Journal of Pharmacy and Pharmacology, 55 :1629-1633. [DOI] [Full-Text] | |
| 2003 | Deegan, O; Walshe, K; Kavanagh, K; Doyle, S (2003) 'Quantitative detection of C-reactive protein using phosphocholine-labelled enzyme or microspheres'. Analytical Biochemistry, 312 :175-181. [Full-Text] | |
| 2003 | Daly, P; Doyle, S (2003) 'The development of a competitive PCR-ELISA for the detection of equine herpesvirus-1'. Journal of Virological Methods, 107 :237-244. [Full-Text] | |
| 2003 | Daly P and Doyle S (2003) 'Development of a Competitive PCR-ELISA for the detection of Equine Herpesvirus-1 (EHV-1)'. Journal of Virological Methods, 107 :237-244. [Full-Text] | |
| 2003 | Dalton, J.P., O'Neill, S.M., Stack, C., Collins, P., Walsh, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moiré, N., Brennan, G.P., Mousley, A., Kreshchenko, N., Maule, A.G. and Donnelly, S.M. (2003) 'Fasciola hepatica cathepsin L-like proteases: biology, function, and potential in the development of first generation liver fluke vaccines'. International Journal For Parasitology, 33 :1173-1181. [Full-Text] | |
| 2003 | Corcoran, A; Mahon, BP; McParland, P; Davoren, A; Doyle, S (2003) 'Ex vivo cytokine responses against parvovirus B19 antigens in previously infected pregnant women'. Journal of Medical Virology, 70 :475-480. [DOI] [Full-Text] | |
| 2002 | Saldanha J.; Lelie N.; Yu M.; Heath A.; Albrecht J.; Buhr S.; Roth W.; Chaput C.; Chudy ; Schmidt I.; Cohen B.; Defer C.; Lefebvre C.; Doyle S.; Daly P.; Echevarria J.; Erdman D.; Anderson B.; Fang R.; Johnstone D.; Gierman T.; Gross P.; Held I.; Jarvis L.; Cleland A.; Lazo A.; Gibaja V.; Murozuka T.; Okada Y.; Pedada L.; Lou A.; Pisani ; Gentili ; Saldanha ; Stolz M.; Thomas I.; Mathy E.; Weimer T.; Yu M.; Shen L.; Mason B.; York D.; Zhang H. (2002) 'Establishment of the first World Health Organization International Standard for human parvovirus B19 DNA nucleic acid amplification techniques'. Vox Sanguinis, 82 (1):24-31. [DOI] [Full-Text] | |
| 2002 | Daly, P; Collier, T; Doyle, S (2002) 'PCR-ELISA detection of Escherichia coli in milk'. Letters in Applied Microbiology, 34 :222-226. [Full-Text] | |
| 2002 | Raab U, Beckenlehner K, Lowin T, Niller H-H, Doyle S and Modrow S (2002) 'NS1protein of Parvovims B19 interacts directly and via the transcription factors Spl/Sp3 with the p6 promoter'. 293 :86-93. [Full-Text] | |
| 2002 | Saldanda J, Lelie N, Yu MW and B19 Collaborative Study Group (incl. Doyle S and Daly P) (2002) 'Establishment of the first World Health Organisation International Standard for human parvovirus B19 DNA nucleic acid amplification techniques'. Vox Sanguinis, 82 :24-31. [Full-Text] | |
| 2002 | Daly P.; Corcoran A.; Mahon B.; Doyle S. (2002) 'High-sensitivity PCR detection of parvovirus B19 in plasma'. Journal of Clinical Microbiology, 40 (6):1958-1962. [DOI] [Full-Text] | |
| 2002 | Raab, U; Beckenlehner, K; Lowin, T; Niller, HH; Doyle, S; Modrow, S (2002) 'NS1 protein of parvovirus B19 interacts directly with DNA sequences of the P6 promoter and with the cellular transcription factors Sp1/Sp3'. Virology, 293 :86-93. [DOI] [Full-Text] | |
| 2001 | Ennis, O; Corcoran, A; Kavanagh, K; Mahon, BP; Doyle, S (2001) 'Baculovirus expression of parvovirus B19 (B19V) NS1: utility in confirming recent infection'. Journal of Clinical Virology, 22 :55-60. [Full-Text] | |
| 2000 | Doyle, S; Kerr, S; O'Keeffe, G; O'Carroll, D; Daly, P; Kilty, C (2000) 'Detection of parvovirus B19 IgM by antibody capture enzyme immunoassay: receiver operating characteristic analysis'. Journal of Virological Methods, 90 :143-152. [Full-Text] | |
| 2000 | Corcoran A, Doyle S, Waldron D, Nicholson A and Mahon BP. (2000) 'Recently infected children display impaired interferon-y responses against Parvovirus B19. 8th Annual Congress of the British Society for Immunology, Harrogate, UK, December 2000'. 101 . | |
| 2000 | Cotter, G; Doyle, S; Kavanagh, K (2000) 'Development of an insect model for the in vivo pathogenicity testing of yeasts'. FEMS Immunology and Medical Microbiology, 27 :163-169. [Full-Text] | |
| 2000 | Corcoran A; Doyle S; Waldron D; Nicholson A; Mahon BP; (2000) 'Impaired gamma interferon responses against parvovirus B19 by recently infected children'. Virology, 74 (21). [Full-Text] | |
| 1999 | Eckersall, PD; Duthie, S; Safi, S; Moffatt, D; Horadagoda, NU; Doyle, S; Parton, R; Bennett, D; Fitzpatrick, JL (1999) 'An automated biochemical assay for haptoglobin: Prevention of interference from albumin'. Comparative Haematology International, 9 :117-124. [Full-Text] | |
| 1999 | Kerr, S; O'Keeffe, G; Kilty, C; Doyle, S (1999) 'Undenatured parvovirus B19 antigens are essential for the accurate detection of parvovirus B19 IgG'. Journal of Medical Virology, 57 :179-185. [Full-Text] | |
| 1999 | Flendrig L, Chamuleau RAFM, Maas MAW, Daalhuisen J, Hassett B, Kilty CJ, Doyle S, Ladiges NCJJ, Jorning GGA, la Soe JW, Sommeijer D and te Velde AA. (1999) 'Evaluation of a novel design bioartificial liver in rats with complete liver ischemia: treatment efficacy and species-specific a-GST detection as a first attempt to monitor hepatocyte viability'. 30 :311-321. [Full-Text] | |
| 1998 | Kilty C.; Doyle S.; Hassett B.; Manning F. (1998) 'Glutathione S-transferases as biomarkers of organ damage: Applications of rodent and canine GST enzyme immunoassays'. Chemico-Biological Interactions, 111-112 :123-135. [DOI] [Full-Text] | |
| 1997 | Clarke, H, Egan, DA., Heffernan, M, Doyle, S, Byrne, C, Kilty, C and Ryan, MP (1997) 'Alpha-glutathione s-transferase (alpha-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat'. HUMAN EXPERIMENTAL TOXICOLOGY, 16 :154-157. [Full-Text] | |
| 1996 | Hobson, AH, McCormack, C, Doyle, S, Jackson, J, Kilty, C and Whitehead, A (1996) 'Generation of soluble recombinant human acute phase serum amyloid A2 protein and its use in the development of an A-SAA specific ELISA'. Journal of Immunological Methods, 198 :101-110. [Full-Text] | |
| 1995 | Rees, GW, Trull, AK and Doyle, S (1995) 'Evaluation of an enzyme-immunometric assay for serum glutathione s-transferase'. Annals of Clinical Biochemistry, 32 :575-583. [Full-Text] | |
| 1990 | Doyle JM, Schinina ME, Bossa F, Doonan S (1990) 'The amino-acid-sequence of cytosolic aspartate-aminotransferase from human liver'. Biochemical Journal, 270 :651-657. [Full-Text] |
Book Chapter
| Year | Publication | |
|---|---|---|
| 2017 | Doyle S. and Owens R.A. (2017) 'Fungal Proteomics' In: Fungi: Biology and Applications, 3rd Edition. United Kingdom : Wiley Blackwell. [Link] | |
| 2014 | Owens, RA; O' Keeffe, G; O' Hanlon, KA; Gallagher, L; Doyle, S (2014) 'Virulence Characteristics of Aspergillus fumigatus' In: HUMAN PATHOGENIC FUNGI: MOLECULAR BIOLOGY AND PATHOGENIC MECHANISMS. WYMONDHAM : CAISTER ACADEMIC PRESS. | |
| 2011 | Doyle, S (2011) 'Fungal Proteomics' In: FUNGI: BIOLOGY AND APPLICATIONS, 2ND EDITION. OXFORD : BLACKWELL SCIENCE PUBL. | |
| 2011 | O'Shaughnessy, L; Doyle, S (2011) 'Purification of Proteins from Baculovirus-Infected Insect Cells' In: PROTEIN CHROMATOGRAPHY: METHODS AND PROTOCOLS. TOTOWA : HUMANA PRESS INC. [DOI] | |
| 2011 | Doyle, S. (2011) 'The Detection of Parvoviruses' In: Methods in Molecular Biology- Diagnostic Virology Protocols. : Humana Press. [DOI] [Full-Text] | |
| 2010 | Doyle S. (2010) 'Nonribosomal Peptide Synthesis' In: Amino Acids, Peptides and Proteins in Organic Chemistry. [DOI] | |
| 2007 | Corcoran, A; Doyle, S (2007) 'Human Parvovirus B19: Molecular Virology, Clinical Features, Prevalence, Diagnosis and Control' In: CONGENITAL AND OTHER RELATED INFECTIOUS DISEASES OF THE NEWBORN. AMSTERDAM : ELSEVIER SCIENCE BV. [DOI] [Full-Text] | |
| 1996 | C.G. Kilty and S. Doyle (1996) 'Alpha Glutathione s-transferases as biomarkers of organ viability and damage' In: NPE Vermeulen, GJ Mulder, Nieuwenhuyse WHM Peters and PJ van Bladeren(Eds.). Glutathione s-Transferases: Structure, Function and Clinical Implications. London : Taylor & Francis Limited. |
Other Journal
Conference Publication
| Year | Publication | |
|---|---|---|
| 2009 | Lynch, E.M., McGee, M., Doyle, S. & Earley, B. (2009) Proc. Agric. Res. Forum Effect of abrupt weaning and subsequent housing on leukocyte populations and acute phase proteins in beef suckler cows | |
| 2009 | Lynch, E.M., McGee, M., Doyle, S. & Earley, B. (2009) Proc. Agric. Res. Forum Effect of abrupt weaning on circulating leukocyte subsets and phagocytic activity of neutrophils in beef suckler calves | |
| 2007 | Carberry, S; Doyle, S (2007) CYTOTECHNOLOGY Proteomic studies in biomedically and industrially relevant fungi [DOI] [Full-Text] | |
| 2003 | Dalton J.; Neill S.; Stack C.; Collins P.; Walshe A.; Sekiya M.; Doyle S.; Mulcahy G.; Hoyle D.; Khaznadji E.; Moiré N.; Brennan G.; Mousley A.; Kreshchenko N.; Maule A.; Donnelly S. (2003) International Journal for Parasitology Fasciola hepatica cathepsin L-like proteases: Biology, function, and potential in the development of first generation liver fluke vaccines [DOI] [Full-Text] | |
| 1998 | Kilty, C; Doyle, S; Hassett, B; Manning, F (1998) CHEMICO-BIOLOGICAL INTERACTIONS Glutathione S-transferases as biomarkers of organ damage: applications of rodent and canine GST enzyme immunoassays [Full-Text] |
Conference Contribution
Book Review
| Year | Publication | |
|---|---|---|
| 2011 | O'Shaughnessy, Luke and Doyle, Sean (2011) Purification of proteins from baculovirus-infected insect cells. [Book Review] [DOI] |
Editorial
| Year | Publication | |
|---|---|---|
| 2015 | Medema, MH; Kottmann, R; Yilmaz, P; Cummings, M; Biggins, JB; Blin, K; de Bruijn, I; Chooi, YH; Claesen, J; Coates, RC; Cruz-Morales, P; Duddela, S; Dusterhus, S; Edwards, DJ; Fewer, DP; Garg, N; Geiger, C; Gomez-Escribano, JP; Greule, A; Hadjithomas, M; Haines, AS; Helfrich, EJN; Hillwig, ML; Ishida, K; Jones, AC; Jones, CS; Jungmann, K; Kegler, C; Kim, HU; Kotter, P; Krug, D; Masschelein, J; Melnik, AV; Mantovani, SM; Monroe, EA; Moore, M; Moss, N; Nutzmann, HW; Pan, GH; Pati, A; Petras, D; Reen, FJ; Rosconi, F; Rui, Z; Tian, ZH; Tobias, NJ; Tsunematsu, Y; Wiemann, P; Wyckoff, E; Yan, XH; Yim, G; Yu, FG; Xie, YC; Aigle, B; Apel, AK; Balibar, CJ; Balskus, EP; Barona-Gomez, F; Bechthold, A; Bode, HB; Borriss, R; Brady, SF; Brakhage, AA; Caffrey, P; Cheng, YQ; Clardy, J; Cox, RJ; De Mot, R; Donadio, S; Donia, MS; van der Donk, WA; Dorrestein, PC; Doyle, S; Driessen, AJM; Ehling-Schulz, M; Entian, KD; Fischbach, MA; Gerwick, L; Gerwick, WH; Gross, H; Gust, B; Hertweck, C; Hofte, M; Jensen, SE; Ju, JH; Katz, L; Kaysser, L; Klassen, JL; Keller, NP; Kormanec, J; Kuipers, OP; Kuzuyama, T; Kyrpides, NC; Kwon, HJ; Lautru, S; Lavigne, R; Lee, CY; Linquan, B; Liu, XY; Liu, W; Luzhetskyy, A; Mahmud, T; Mast, Y; Mendez, C; Metsa-Ketela, M; Micklefield, J; Mitchell, DA; Moore, BS; Moreira, LM; Muller, R; Neilan, BA; Nett, M; Nielsen, J; O'Gara, F; Oikawa, H; Osbourn, A; Osburne, MS; Ostash, B; Payne, SM; Pernodet, JL; Petricek, M; Piel, J; Ploux, O; Raaijmakers, JM; Salas, JA; Schmitt, EK; Scott, B; Seipke, RF; Shen, B; Sherman, DH; Sivonen, K; Smanski, MJ; Sosio, M; Stegmann, E; Sussmuth, RD; Tahlan, K; Thomas, CM; Tang, Y; Truman, AW; Viaud, M; Walton, JD; Walsh, CT; Weber, T; van Wezel, GP; Wilkinson, B; Willey, JM; Wohlleben, W; Wright, GD; Ziemert, N; Zhang, CS; Zotchev, SB; Breitling, R; Takano, E; Glockner, FO (2015) Minimum Information about a Biosynthetic Gene cluster. NEW YORK: [Editorial] [Full-Text] |
Magazine Article
Reviews
| Year | Publication | |
|---|---|---|
| 2015 | Sheridan K.; Dolan S.; Doyle S. (2015) Endogenous cross-talk of fungal metabolites. [Reviews] [DOI] [Full-Text] | |
| 2015 | Dolan, SK; O'Keeffe, G; Jones, GW; Doyle, S (2015) Resistance is not futile: gliotoxin biosynthesis, functionality and utility. OXFORD: [Reviews] [DOI] [Full-Text] | |
| 2014 | Johnson, G; Ferrini, A; Dolan, SK; Nolan, T; Agrawal, S; Doyle, S; Bustin, SA (2014) Biomarkers for invasive aspergillosis: the challenges continue. LONDON: [Reviews] [DOI] [Full-Text] | |
| 2011 | Doyle, S (2011) Fungal proteomics: from identification to function. HOBOKEN: [Reviews] [DOI] [Full-Text] | |
| 2007 | Stack, D; Neville, C; Doyle, S (2007) Nonribosomal peptide synthesis in Aspergillus fumigatus and other fungi. READING: [Reviews] [DOI] [Full-Text] | |
| 2006 | Corcoran A.; Doyle S. (2006) Human Parvovirus B19: Molecular Virology, Clinical Features, Prevalence, Diagnosis and Control. [Reviews] [DOI] [Full-Text] | |
| 2004 | Corcoran, A; Doyle, S (2004) Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19. READING: [Reviews] [DOI] [Full-Text] |
Letters
| Year | Publication | |
|---|---|---|
| 2005 | Corcoran A.; Doyle S.; Allain J.; Candotti D.; Parsyan A. (2005) Evidence of serological cross-reactivity between genotype 1 and genotype 3 erythrovirus infections (multiple letters). [Letters] [DOI] [Full-Text] |
Publication Letters
| Year | Publication | |
|---|---|---|
| 2005 | Corcoran, A; Doyle, S (2005) Evidence of serological cross-reactivity between genotype 1 and genotype 3 erythrovirus infections. WASHINGTON: [Publication Letters] [DOI] [Full-Text] |
Certain data included herein are derived from the © Web of Science (2024) of Clarivate. All rights reserved.
Teaching Interests
Module Contribution: 18 per annum over the last 7 years (2017-2024) across Seven BSc degree programmes: Biotechnology, Biological and Biomedical Science, Single and Double Honours Biology, Pharmaceutical and Biomedical Chemistry and Science Education.
Year 1: BI103 Literature Projects; BI108 Oral Presentations.
Year 2/3: BI305 Dissertation; BI308 Proteomics; BI309 General Methodology and BI314* Scientific Communication.
Year 4: BI411* Bioethics and Biotechnology; BI415* Bioprocessing II; BI420 Seminar Series; BI423 Dissertation; BI424 Literature Project (Double Honours); BI425 Professional module (Patenting & Licensing); BI428 Laboratory Project (Double Honours); BI430 Outplacement (Biotechnology); BI445 Dissertation and Laboratory Project (Biomedical Science) and BI433 Translational Clinical Research.
MSc: BI609 Laboratory Project, MHD02.
PhD: MHD04.
* Module Coordinator, plus BI412 Bioethics and Biobusiness.
BSc (Biotechnology) Degree Coordinator (1997-2023):
Coordinated until 2023 by Professor Sean Doyle, the BSc (Biotechnology) degree (CAO code: MH202) at Maynooth University was introduced in 1996 by Head of Department, Professor Peter Whittaker, and the first students graduated in 2000. Since then, over 470 students have graduated with a BSc (Biotechnology) from Maynooth University. Numerous teaching methodologies are utilized for the programme combining didactic, self-directed and active learning, and include lectures and practical classes, allied to experiential learning in terms of Industry-based educational outplacement (3 months in final year), and acquisition of presentation skills and site visits to biotechnology companies. The degree is of four-year duration, requires simultaneous study of Chemistry for 3 of the 4 years, and is one of the most popular and highest points courses in the Faculty of Science and
Engineering. The course has been modularised and semesterised since 2003.
Annually, it is actively promoted by course co-ordinator Professor Doyle, in
association with colleagues in the University Admissions Office. In academic
year 2018-19, there are 95 students taking Biotechnology at Maynooth University
(years 1-4). Students take 4 subjects in 1st year, three in 2nd
year, two in 3rd year and only Biotechnology in 4th year.
Indeed, in 4th year, all lecture modules are completed in Semester 1 and
students go on work placement during Semester 2- with modules being examined at the end of both Semester 1 and 2, respectively. It was the first Maynooth
University degree programme to offer an industrial placement, which served as a
predicate for many of the industrial placement modules now found in other
Maynooth University degrees (2018). Professor Doyle managed the industrial
outplacement programme until 2015, and the BSc (Biotechnology) was used as a
basis to establish the BSc (Biomedical and Pharmaceutical Chemistry) degree at
Maynooth University, indeed both courses still share high percentage identity
for years 1 to 3. Dr David Fitzpatrick and Dr Paul Dowling now lead the
industrial placement of 4th year Biotechnology students, with
support from Professor Doyle. The course has evolved since its inception and
now places a strong emphasis on both molecular biotechnology and chemical
biology, in particular the identification, study and use of biomolecules as
diagnostic and therapeutic agents. Students taking the BSc (Biotechnology)
programme experience both subject-unique modules and shared modules with other Departmental undergraduates, and have found employment in the
biopharmaceutical, pharmaceutical, diagnostic and food industries. Many BSc
(Biotechnology) graduates have undertaken PhD degrees at Maynooth University
and elsewhere, and are now in both academic and industrial leadership
positions.