Maynooth University Department of Biology

Mass Spectrometry
Mass spectrometry facilities including a high resolution quantitative mass spectrometer (Thermo Fisher Q-Exactive) and an ion trap (Agilent 6340) are available in the Department of Biology, Maynooth University. This facility is jointly funded by Science Foundation Ireland and HEA. External users, both academic and industrial are welcome to use this facility at commercial rates.
Services include

• Protein identification in complex mixtures
• Relative abundance of protein comparator samples
• Biomarker identification
• Small molecule analysis and identification

List of peer-reviewed publications arising from use of the facility:

• SFI-funded-Q-Exactive Mass Spectrometry Publications

Contact:  Caroline Batchelor - email: [email protected]
Location: Callan Building, Maynooth University Department of Biology​

CryoViz Whole Body Cryo-Imaging System
 
Purpose of the equipment: The CryoViz Whole body cryo-imaging system allows imaging of fluorescent-labelled molecules and cells in the context of an entire organism. This approach provides a combination of field of view, depth of field, resolution and colour and fluorescence contrast mechanisms which are simply not possible with non-invasive in vivo imaging or with traditional ex vivo microscope imaging approaches.
 
Technical information/equipment spec: Automated sectioning (range 5-200 um) and imaging, XYZ robotic zoom microscope enabling image tiling. 3D visualization software.
 

3D visualization of stem cell bio-distribution in a whole body using the CryoVizTM cryo-imaging system from BioInVision. This novel technology finds many applications in research on regenerative medicine, cancer, gene expression, toxicology, phenotype, molecular biology, drug discovery, etc.
 
Contact: Ica Dix – email: [email protected]
Location: Biosciences Building, Maynooth University Department of Biology

 

Olympus FluoView1000 laser scanning confocal microscope

Purpose of this equipment: high resolution images of  living  and fixed cells and tissues

Technical information/equipment spec: Olympus IX81 inverted microscope, with six laser lines (405nm, 458nm, 488nm, 515nm, 543nm, 633nm ) and spectral detection system.
Applications: high resolution confocal images, 3D rendering and animation, spectral unmixing, spectral fingerprinting (lambda stack), co-localisation, time lapse experiments,

Contact: Ica Dix - [email protected]
Location: Bioscience Building, Maynooth University Department of Biology

Purpose of the equipment: The Attune NxT flow cytometer allows the analysis of up to 14 fluorescent parameters on cell populations. The Attune NxT utilises acoustic assisted hydrodynamic focusing technology enhancing sample throughput rates.
 
Technical information/equipment spec: The Attune Nxt has 4 lasers (Blue/red/yellow/violet) with a total of 16 detection channels. The Autosampler allows the option to run samples (96 or 384 well plates) in a high throughput fashion. The Attune NxT software enables user defined experimental analysis and the data can also be analysed using standard software including FlowJo.
 
Financial support for the Attune NxT was provided to Maynooth University Department of biology by Science Foundation Ireland (SFI) under Grant Number 16/RI/3399.  

 
Key Responsible Investigator: Karen English: [email protected]
Location: Biosciences Building, Maynooth University Department of Biology

Alphascreen technology-certified multimode plate reader

Purpose of the equipment:  The Clariostar Alphascreen-certified plate reader gives researchers from academia and industry access to Alphascreen® technology, a recently developed bead-based technology, that enables the semi-quantitative measurement of protein-protein and protein-nucleic acid interactions in multiwell format for a rapid screening of conditions and/or small molecules that modulate these interactions. Assays can be carried out using either purified components or in cell extracts. In addition, the Alphascreen-certified plate reader can be used for other high-throughput assays that require sensitive absorbance, luminescence or fluorescence measurements, such as the high-throughput real-time monitoring of enzymatic activities or bacterial growth in multiwell plates.

Technical Information/Equipment Spec:  Continuously adjustable bandwidths (8 to 100 nm) and wavelengths (320 to 850 nm); Combined use of LVF monochromatorsTM and filters in the same measurement; Full-spectrum UV/Vis absorbance measurements in less than 1 second; Dedicated, high-energy laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM; Automated focal Z-height adjustment for top and bottom readings; Includes multi-user control and MARS data analysis software with integrated fluorophore library.
 

Key responsible investigator:  Dr. Martina Schroeder:  [email protected]

Location:  Bioscience Building, Maynooth University, 1st floor