Dr Emmanuelle Graciet
Biology, Human Health Institute, Faculty of Science & Engineering
Associate Professor
Faculty of Science and Engineering Secretary
Biography
2000: MSc in Plant Molecular and Cellular Physiology (Institut National Agronomique Paris-Grignon (now AgroParisTech)), France
2003: PhD in Biochemistry (University Pierre and Marie Curie (Paris 6)), France
2003-2006: postdoctoral fellow (Caltech), USA
2006-2009: postdoctoral fellow (Trinity College Dublin)
2009-2013: SFI-SIRG principal investigator (Trinity College Dublin)
2003: PhD in Biochemistry (University Pierre and Marie Curie (Paris 6)), France
2003-2006: postdoctoral fellow (Caltech), USA
2006-2009: postdoctoral fellow (Trinity College Dublin)
2009-2013: SFI-SIRG principal investigator (Trinity College Dublin)
2014-2020: Assistant Professor/Lecturer (Maynooth University)
From 2020: Associate Professor (Maynooth University)
Research Interests
Research in the Plant Biochemistry Lab focuses on the role of protein degradation mediated by the ubiquitin system in the regulation of plant responses to biotic and abiotic stresses and to combinations of stresses, with a focus on waterlogging/hypoxia and pathogens.
For this work, we use the model plant Arabidopsis thaliana, but also crops such as barley (Hordeum vulgare) and oilseed rape (Brassica napus), in combination with biochemical, molecular, genetic and genomics approaches. More specifically, we aim at understanding how the nature of the N-terminal residue of proteins affects their stability and participates to the regulation of plant-pathogen interactions.
Another aspect of our work aims at understanding how plants respond to combinations of environmental stresses, with a focus on waterlogging (and the resulting hypoxia) and pathogens. The Plant Biochemistry Lab is a member of:
- the Virtual Irish Centre for Crop Improvement (VICCI). Additional details available at: http://vicci.ie/
- the Sustainable Ecosystems Research Group at Maynooth University
Peer Reviewed Journal
| Year | Publication | |
|---|---|---|
| 2024 | Brian C. Mooney; Catherine M. Doorly; Melissa Mantz; Pablo Garcia; Pitter Huesgen; Emmanuelle Graciet (2024) 'Hypoxia represses pattern-triggered immune responses in Arabidopsis'. PLANT PHYSIOLOGY, . | |
| 2024 | Garcia, P.; Singh, S.; Graciet, E. (2024) 'New Insights into the Connections between Flooding/Hypoxia Response and Plant Defenses against Pathogens'. Plants (Basel, Switzerland), 13 . [DOI] | |
| 2023 | Zhang, H; Rundle, C; Winter, N; Miricescu, A; Mooney, BC; Bachmair, A; Graciet, E; Theodoulou, FL (2023) 'BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition'. The Plant cell, . [DOI] | |
| 2023 | Brazel, A.J.; Graciet, E. (2023) 'Complexity of Abiotic Stress Stimuli: Mimicking Hypoxic Conditions Experimentally on the Basis of Naturally Occurring Environments'. Methods in molecular biology (Clifton, N.J.), 2642 . [Link] [DOI] | |
| 2023 | Miricescu, A.; Brazel, A.J.; Beegan, J.; Wellmer, F.; Graciet, E. (2023) 'Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response'. Plant Direct, 7 . [Link] [DOI] [Full-Text] | |
| 2022 | Byrne T.; Grant J.; Kock-Appelgren P.; Förster L.; Michel T.; Miricescu A.; Thomas W.T.B.; Graciet E.; Spink J.; Ng C.K.Y.; Barth S. (2022) 'Improving phenotyping in winter barley cultivars towards waterlogging tolerance by combining field trials under natural conditions with controlled growth condition experiments'. European Journal of Agronomy, 133 . [DOI] [Full-Text] | |
| 2021 | Doorly C.M.; Graciet E. (2021) 'Lessons from comparison of hypoxia signaling in plants and mammals'. Plants (Basel, Switzerland), 10 (5). [DOI] [Full-Text] | |
| 2021 | Miricescu A.; Byrne T.; Doorly C.M.; Ng C.K.Y.; Barth S.; Graciet E. (2021) 'Experimental comparison of two methods to study barley responses to partial submergence'. Plant Methods, 17 (1). [DOI] [Full-Text] | |
| 2021 | Mooney BC; Mantz M; Graciet E; Huesgen PF; (2021) 'Cutting the line: manipulation of plant immunity by bacterial type III effector proteases'. Journal of Experimental Botany, . [DOI] [Full-Text] | |
| 2020 | Mooney B.C.; Graciet E. (2020) 'A simple and efficient Agrobacterium-mediated transient expression system to dissect molecular processes in Brassica rapa and Brassica napus'. Plant Direct, 4 (7). [DOI] [Full-Text] | |
| 2019 | Goslin K; Eschen-Lippold L; Naumann C; Linster E; Sorel M; Klecker M; de Marchi R; Kind A; Wirtz M; Lee J; Dissmeyer N; Graciet E; (2019) 'Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease'. Plant Physiology, 180 :2272-2289. [DOI] [Full-Text] | |
| 2018 | Miricescu A; Goslin K; Graciet E; (2018) 'Ubiquitylation in plants: signaling hub for the integration of environmental signals'. Journal of Experimental Botany, 69 (19):4511-4527. [DOI] [Full-Text] | |
| 2018 | Dissmeyer N.; Rivas S.; Graciet E. (2018) 'Life and death of proteins after protease cleavage: protein degradation by the N-end rule pathway'. New Phytologist, 218 (3):929-935. [DOI] [Full-Text] | |
| 2017 | Dissmeyer N; Graciet E; Holdsworth MJ; Gibbs DJ; (2017) 'N-term 2017: Proteostasis via the N-terminus'. Trends in Biochemical Sciences, 44 :293-295. [DOI] [Full-Text] | |
| 2017 | Goslin K; Zheng B; Serrano-Mislata A; Rae L; Ryan PT; Kwaśniewska K; Thomson B; O'Maoileidigh D; Madueno F; Wellmer F; Graciet E; (2017) 'Transcription factor Interplay between LEAFY and APETALA1/ CAULIFLOWER during Floral Initiation'. Plant Physiology, 174 :1097-1109. [DOI] [Full-Text] | |
| 2017 | Serrano-Mislata A; Goslin K; Zheng B; Rae L; Wellmer F; Graciet E; Madueño F; (2017) 'Regulatory interplay between LEAFY, APETALA1/CAULIFLOWER and TERMINAL FLOWER1: New insights into an old relationship'. Plant Signaling and Behavior, 12 . [DOI] [Full-Text] | |
| 2016 | de Marchi R; Sorel M; Mooney B; Fudal I; Goslin K; Kwaśniewska K; Ryan PT; Pfalz M; Kroymann J; Pollmann S; Feechan A; Wellmer F; Rivas S; Graciet E; (2016) 'The N-end rule pathway regulates pathogen responses in plants'. Scientific Reports, 6 . [DOI] [Full-Text] | |
| 2015 | O'Maoileidigh, DS; Thomson, B; Raganelli, A; Wuest, SE; Ryan, PT; Kwasniewska, K; Carles, CC; Graciet, E; Wellmer, F (2015) 'Gene network analysis of Arabidopsis thaliana flower development through dynamic gene perturbations'. Plant Journal, 83 :344-358. [DOI] [Full-Text] | |
| 2015 | Ryan, PT; O'Maoileidigh, DS; Drost, HG; Kwasniewska, K; Gabel, A; Grosse, I; Graciet, E; Quint, M; Wellmer, F (2015) 'Patterns of gene expression during Arabidopsis flower development from the time of initiation to maturation'. BMC Genomics, 16 . [DOI] [Full-Text] | |
| 2014 | D. Ó’Maoiléidigh, E. Graciet and F. Wellmer (2014) 'Gene networks controlling flower development'. New Phytologist, 201 :16-30. [Full-Text] | |
| 2014 | Wellmer, F; Graciet, E; Riechmann, JL (2014) 'Specification of floral organs in Arabidopsis'. Journal of Experimental Botany, 65 :1-9. [DOI] [Full-Text] | |
| 2013 | Piatkov K; Graciet E; Varshavsky A; (2013) 'Ubiquitin reference technique and its use in ubiquitin-lacking prokaryotes'. PLoS ONE, 8 (6). [DOI] [Full-Text] | |
| 2013 | D. Ó’Maoiléidigh, S. E. Wuest, L. Rae, P. T. Ryan, K. Kwaśniewska, P. Das, A. Lohan, B. Loftus, E. Graciet*, and F. Wellmer (2013) 'Interplay of B and C function regulators in the control of floral organ development'. Plant Cell, 25 :2482-2503. [Full-Text] | |
| 2012 | S. E. Wuest, D. ӒMaoilidigh, L. Rae, K. Kwaśniewska, A. Raganelli, K. Hanczaryk, A. Lohan, B. Loftus, E. Graciet, and F. Wellmer (2012) 'Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 109 :13452-13457. [Full-Text] | |
| 2010 | E. Graciet*, F. Mesiti and F. Wellmer* (2010) 'Structure and evolutionary conservation of the plant N-end rule pathway'. Plant Journal, 61 :741-751. [Full-Text] | |
| 2010 | E. Graciet and F. Wellmer (2010) 'The plant N-end rule pathway: structure and functions'. Trends in Plant Science, 15 :447-453. [Full-Text] | |
| 2009 | E. Graciet*, F. Walter, D. OMaoilidigh, S. Pollmann, E. M. Meyerowitz, A. Varshavsky and F. Wellmer* (2009) 'The N-end rule pathway controls multiple functions during Arabidopsis shoot and leaf development'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 106 :13618-13623. [Full-Text] | |
| 2006 | Lebreton S; Andreescu S; Graciet E; Gontero B; (2006) 'Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii. Functional consequences for glyceraldehyde-3-phosphate dehydrogenase'. FEBS Journal, 273 (14):3358-3369. [DOI] [Full-Text] | |
| 2006 | E. Graciet, R-G. Hu, K. Piatkov, J.H. Rhee, E. M. Schwarz and A. Varshavsky (2006) 'Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen'. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, 103 :3078-3083. [Full-Text] | |
| 2005 | Delobel A.; Graciet E.; Andreescu S.; Gontero B.; Halgand F.; Laprévote O. (2005) 'Rapid Communications in Mass Spectrometry'. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 19 (22):3379-3388. [DOI] [Full-Text] | |
| 2004 | E. Graciet, S. Lebreton, and B. Gontero (2004) 'Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex'. Journal of Experimental Botany, 55 :1245-1254. [Full-Text] | |
| 2004 | E. Graciet, G. Mulliert, S. Lebreton, and B. Gontero (2004) 'Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation'. European Journal of Biochemistry, 271 :4737-4744. [Full-Text] | |
| 2003 | Graciet, E., Gans, P., Wedel, N., Lebreton, S., Camadro, J.-M., Gontero, B. (2003) 'The small protein CP12: A protein linker for supramolecular complex assembly'. 42 (27):8163-8170. [Link] [DOI] [Full-Text] | |
| 2003 | S. Lebreton, E. Graciet, and B. Gontero (2003) 'Modulation, via protein-protein interactions, of glyceraldehyde-3-phosphate dehydrogenase activity through redox phosphoribulokinase regulation'. Journal of Biological Chemistry, 278 :12078-12084. [Full-Text] | |
| 2003 | E. Graciet, S. Lebreton, J-M. Camadro, and B. Gontero (2003) 'Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for conformation changes upon association with the small protein CP12'. European Journal of Biochemistry, 270 :129-136. [Full-Text] | |
| 2000 | Wessel, P.M., Graciet, E., Douce, R., Dumas, R. (2000) 'Evidence for two distinct effector-binding sites in threonine deaminase by site-directed mutagenesis, kinetic, and binding experiments'. 39 (49):15136-15143. [Link] [DOI] [Full-Text] |
Book Chapter
| Year | Publication | |
|---|---|---|
| 2017 | Thomson B; Graciet E; Wellmer F; (2017) 'Inducible Promoter Systems for Gene Perturbation Experiments in Arabidopsis' In: Methods in molecular biology. USA : Clifton, N.J. [DOI] | |
| 2016 | Stewart D; Graciet E; Wellmer F; (2016) 'Molecular and regulatory mechanisms controlling floral organ development' In: [DOI] | |
| 2014 | Graciet E; O'Maoiléidigh DS; Wellmer F; (2014) 'Next-generation sequencing applied to flower development: ChIP-Seq' In: Methods in molecular biology. USA : Clifton N.J. [DOI] [Full-Text] | |
| 2014 | Wellmer F; Bowman JL; Davies B; Ferrándiz C; Fletcher JC; Franks RG; Graciet E; Gregis V; Ito T; Jack TP; Jiao Y; Kater MM; Ma H; Meyerowitz EM; Prunet N; Riechmann JL; (2014) 'Flower development: open questions and future directions' In: Methods in Molecular Biology - Flower Development. USA : Springer. [DOI] | |
| 2014 | O'Maoileidigh, DS; Graciet, E; Wellmer, F (2014) 'Genetic Control of Arabidopsis Flower Development' In: MOLECULAR GENETICS OF FLORAL TRANSITION AND FLOWER DEVELOPMENT. LONDON : ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD. [DOI] |
Editorial
| Year | Publication | |
|---|---|---|
| 2022 | Willems P.; Huesgen P.F.; Finkemeier I.; Graciet E.; Meinnel T.; Van Breusegem F. (2022) Editorial: Plant protein termini: Their generation, modification and function. [Editorial] [DOI] |
Certain data included herein are derived from the © Web of Science (2024) of Clarivate. All rights reserved.
Committees
Employment
Teaching Interests
My primary teaching interests
are plant biology and biochemistry.
I lecture in the following modules:
- BI201 - Biochemistry
- BI305 - Literature review, including tutorials
- BI306 - Plant Responses to the Environment
- BI309 - General Methodology
- BI440 - Control of Protein Activity and Abundance
- BI425 - BioPharma - producing human
therapeutics in plants
I am also the second year coordinator and MH201 Deputy course coordinator for the Biology Department.