Dr Sinead Miggin

Biology, Human Health Institute

Lecturer
Assistant Professor

Biosciences
B3.14
(01) 708 3855

Biography

Dr Sinead Miggin graduated in Toxicology from Athlone Institute of Technology. In 2000, she was awarded a PhD in Biochemistry which was undertaken at the Conway Institute, University College Dublin. She subsequently undertook a Postdoctoral Fellowship in Inflammation research at the Conway Institute (2000-2002) followed by a Senior Postdoctoral Fellowship at the Department of Biochemistry and Immunology, Trinity College Dublin, Ireland (2002-2006). She was appointed Assistant Professor in the Department of Biology at Maynooth University in 2006. In 2015, Dr Miggin was awarded a Postgraduate Diploma in Higher Education. Dr Miggin is Principle Investigator in the Immune Signalling lab and is a member of the Kathleen Lonsdale Institute for Human Health Research. 

Research Interests

My research focuses on the molecular mechanisms involved in regulating the innate immune response through the Toll-like receptors (TLRs). TLRs play a critical role in the evolutionary conserved innate immune response and represents our first line of defense against invading pathogens. TLRs are activated in response to a broad spectrum of pathogen-associated molecular patterns (PAMPs) ranging from bacterial and viral components to fungal and protozoal molecules. Chronic Inflammatory Diseases such as Rheumatoid Arthritis (RA), Osteoarthritis (OA) and Type 2 Diabetes, whilst having apparently unrelated aetiologies, share a common late stage pathogenic process, characterized by increases in pro-inflammatory cytokines, such as TNFa, IL-1b, IL-6 and IL-18. During chronic inflammatory episodes, the 'in-born' or innate immune system may become dysregulated through mechanisms involving Toll-like receptors (TLRs). To better understand the inflammatory response, my group employ cutting edge proteomics technologies to identify novel modulators of TLR signalling and inflammatory processes. Thereafter, molecular immunological approaches are adopted to explore the role of newly identified proteins in the context of inflammatory conditions and antiviral immunity. In particular, my group are interested in the pathogenesis of Osteoarthritis and Type II Diabetes. My group focus on the identification and functional characterisation of proteins that modulate inflammatory and antiviral immunity at the molecular level.

Peer Reviewed Journals

  Year Publication
2017 'Analysis of Inflammatory Cytokines in Pre-diabetic subjects'
Gupta, S., Maratha, A, Natarajan, A, Miggin, S and Hoashi, S (2017) 'Analysis of Inflammatory Cytokines in Pre-diabetic subjects'. Current Research in Diabetes and Obesity Journal, 4 (5) [DOI] [IR Link] [Details]
2017 'Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications'
Gupta S;Maratha A;Siednienko J;Natarajan A;Gajanayake T;Hoashi S;Miggin S; (2017) 'Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications'. Scientific Reports, 7 (1) [DOI] [IR Link] [Details]
2017 'Long non-coding RNA AGER-1 functionally upregulates the innate immunity gene AGER and approximates its anti-tumor effect in lung cancer'
Pan Z;Liu L;Nie W;Miggin S;Qiu F;Cao Y;Chen J;Yang B;Zhou Y;Lu J;Yang L; (2017) 'Long non-coding RNA AGER-1 functionally upregulates the innate immunity gene AGER and approximates its anti-tumor effect in lung cancer'. Molecular Carcinogenesis, [DOI] [IR Link] [Details]
2015 'Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammatory and angiogenic mechanisms'
Connolly M.;Rooney P.;McGarry T.;Maratha A.;McCormick J.;Miggin S.;Veale D.;Fearon U. (2015) 'Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammatory and angiogenic mechanisms'. ANNALS OF THE RHEUMATIC DISEASES, [DOI] [IR Link] [Details]
2014 'The TIR-Domain Containing Adaptor TRAM Is Required for TLR7 Mediated RANTES Production'
Shevlin, E;Miggin, SM (2014) 'The TIR-Domain Containing Adaptor TRAM Is Required for TLR7 Mediated RANTES Production'. PLoS ONE, 9 [DOI] [IR Link] [Details]
2013 'TRIF-Mediated TLR3 and TLR4 Signaling Is Negatively Regulated by ADAM15'
Ahmed, S;Maratha, A;Butt, AQ;Shevlin, E;Miggin, SM (2013) 'TRIF-Mediated TLR3 and TLR4 Signaling Is Negatively Regulated by ADAM15'. Journal of Immunology, 190 :2217-2228 [DOI] [Details]
2011 'Absence of MyD88 Results in Enhanced TLR3-Dependent Phosphorylation of IRF3 and Increased IFN-beta and RANTES Production'
Siednienko, J;Gajanayake, T;Fitzgerald, KA;Moynagh, P;Miggin, SM (2011) 'Absence of MyD88 Results in Enhanced TLR3-Dependent Phosphorylation of IRF3 and Increased IFN-beta and RANTES Production'. Journal of Immunology, 186 :2514-2522 [DOI] [IR Link] [Details]
2011 'Targeted Disruption of Nonribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus'
O'Hanlon, KA;Cairns, T;Stack, D;Schrettl, M;Bignell, EM;Kavanagh, K;Miggin, SM;O'Keeffe, G;Larsen, TO;Doyle, S (2011) 'Targeted Disruption of Nonribosomal Peptide Synthetase pes3 Augments the Virulence of Aspergillus fumigatus'. Infection and Immunity, 79 :3978-3992 [DOI] [IR Link] [Details]
2011 'Nuclear Factor kappa B Subunits RelB and cRel Negatively Regulate Toll-like Receptor 3-mediated beta-Interferon Production via Induction of Transcriptional Repressor Protein YY1'
Siednienko, J;Maratha, A;Yang, S;Mitkiewicz, M;Miggin, SM;Moynagh, PN (2011) 'Nuclear Factor kappa B Subunits RelB and cRel Negatively Regulate Toll-like Receptor 3-mediated beta-Interferon Production via Induction of Transcriptional Repressor Protein YY1'. Journal of Biological Chemistry, 286 :44750-44763 [DOI] [Details]
2010 'Cytokine profiling of pre-diabetic patients'
Gupta, S;Maratha, A;Gajanayake, T;Siednienko, J;Natarajan, A;Hoashi, S;Miggin, S (2010) 'Cytokine profiling of pre-diabetic patients'. IRISH JOURNAL OF MEDICAL SCIENCE, 179 :513-513 [Details]
2010 'TLR3-mediated IFN-beta gene induction is negatively regulated by the TLR adaptor MyD88 adaptor-like'
Siednienko, J;Halle, A;Nagpal, K;Golenbock, DT;Miggin, SM (2010) 'TLR3-mediated IFN-beta gene induction is negatively regulated by the TLR adaptor MyD88 adaptor-like'. European Journal of Immunology, 40 :3150-3160 [DOI] [IR Link] [Details]
2007 'NF-kappa B activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1'
Miggin, SM;Palsson-McDermott, E;Dunne, A;Jefferies, C;Pinteaux, E;Banahan, K;Murphy, C;Moynagh, P;Yamamoto, M;Akira, S;Rothwelll, N;Golenbock, D;Fitzgerald, KA;O'Neill, LAJ (2007) 'NF-kappa B activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1'. Proceedings of the National Academy of Sciences of the United States of America, 104 :3372-3377 [DOI] [IR Link] [Details]
2006 'New insights into the regulation of TLR signaling'
Miggin SM, O'Neill LA (2006) 'New insights into the regulation of TLR signaling'. Journal Of Leukocyte Biology, 80 :220-226 [IR Link] [Details]
2003 'Interferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappa'
Wietek, C., Miggin, S. M., Jefferies, C. A. and O’Neill, L.A. (2003) 'Interferon regulatory factor-3-mediated activation of the interferon-sensitive response element by Toll-like receptor (TLR) 4 but not TLR3 requires the p65 subunit of NF-kappa'. JOURNAL OF BIOLOGICAL CHEMISTRY, 278 :50923-50931 [IR Link] [Details]
2002 'Investigation of a functional requirement for isoprenylation by the human prostacyclin receptor'
Miggin, S. M., Lawler, O. A. and Kinsella, B. T. (2002) 'Investigation of a functional requirement for isoprenylation by the human prostacyclin receptor'. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 :1714-1725 [Details]
2002 'Regulation of the extracellular signal regulated protein kinase cascades by the a and b isoforms of the human thromboxane A2 receptor'
Miggin, S. M. and Kinsella, B. T. (2002) 'Regulation of the extracellular signal regulated protein kinase cascades by the a and b isoforms of the human thromboxane A2 receptor'. MOLECULAR PHARMACOLOGY, 61 :817-831 [Details]
2002 'Characterization of the 5' untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the TP isoforms'
Coyle, AT., Miggin, SM. and Kinsella BT. (2002) 'Characterization of the 5' untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by the TP isoforms'. EUROPEAN JOURNAL OF BIOCHEMISTRY, 269 :4058-4073 [IR Link] [Details]
2001 'Protein kinase A dependent phosphorylation of serine 357 of the mouse prostacyclin receptor regulated its coupling to Gs-, Gi- and to Gq- coupled effector signalling'
Lawler, O. A., Miggin, S. M. and Kinsella, B. T. (2001) 'Protein kinase A dependent phosphorylation of serine 357 of the mouse prostacyclin receptor regulated its coupling to Gs-, Gi- and to Gq- coupled effector signalling'. JOURNAL OF BIOLOGICAL CHEMISTRY, 276 :33596-33607 [IR Link] [Details]
2001 'The effects of the statins lovastatin and cerivastatin on signalling by the prostanoid IP-receptor'
Lawler, O. A., Miggin, S. M. and Kinsella, B. T. (2001) 'The effects of the statins lovastatin and cerivastatin on signalling by the prostanoid IP-receptor'. BRITISH JOURNAL OF PHARMACOLOGY, 132 :1639-1649 [IR Link] [Details]
2001 'Thromboxane A(2) receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells'
Miggin, S.M. and Kinsella, BT. (2001) 'Thromboxane A(2) receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells'. BIOCHIMICA ET BIOPHYSICA ACTA, 1539 :147-162 [IR Link] [Details]
1998 'Expression and tissue distribution of the mRNAs encoding the human thromboxane A2 receptor (TP) alpha and beta isoforms'
Miggin, SM and Kinsella, BT. (1998) 'Expression and tissue distribution of the mRNAs encoding the human thromboxane A2 receptor (TP) alpha and beta isoforms'. BIOCHIMICA ET BIOPHYSICA ACTA, 1425 :543-59 [IR Link] [Details]

Book Chapters

  Year Publication
2011 'Toll-like receptor adaptor proteins'
Shevlin, Enda and Miggin, Sinéad M. (2011) 'Toll-like receptor adaptor proteins' In: Encyclopedia of Signaling Molecules. Springer. [Details]
2011 'Toll-like receptor 3'
Butt, Aisha Qasim and Miggin, Sinéad M. (2011) 'Toll-like receptor 3' In: Encyclopedia of Signaling Molecules. Springer. [Details]
2009 'Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells'
Siednienko, J. and Miggin, S.M. (2009) 'Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells' In: Methods in Molecular Biology, Vol. 517. New Jersey: Humana Press Inc. [Details]

Conference Contributions

  Year Publication
2010 Congress on Trauma, Shock, Inflammation and Sepsis.
(2010) Congress on Trauma, Shock, Inflammation and Sepsis. [Oral Presentation], Comparative Analysis of Cytokine Production by Synoviocytes in Response to Toll-Like Receptor Ligan, TSIS 2010 [Details]
2009 Irish Society of Immunology.
(2009) Irish Society of Immunology. [Poster Presentation], TLR3 signalling in synoviocytes is negatively regulated by ATP through inhibition of NF-kB, St James Hospital, Dublin [Details]
2009 Tri-Society Annual Conference.
(2009) Tri-Society Annual Conference. [Poster Presentation], TLR3 signalling in synoviocytes is negatively regulated by extracellular ATP, Lisbon, Portugal [Details]
2007 Biochemical Society, Pattern-recognition receptors in human disease.
(2007) Biochemical Society, Pattern-recognition receptors in human disease. [Poster Presentation], NF-kB activation by MyD88 adapter-like is regulated by Caspase-1’, Cambridge, UK [Details]
2007 International Cytokine Society, Cytokines in health and disease.
(2007) International Cytokine Society, Cytokines in health and disease. [Poster Presentation], NF-kB activation by MyD88 adapter-like is regulated by Caspase-1, San Francisco, CA, USA [Details]

Magazine Article

  Year Publication
2014 Burnt out Diabetes: Cytokines, Adipokines and TLRs Modulation in Type 2 Diabetes.
Gupta, S;Maratha, A;Natarajan, A;Gajanayake, T;Siednienko, J;Hoashi, S;Miggin, S (2014) Burnt out Diabetes: Cytokines, Adipokines and TLRs Modulation in Type 2 Diabetes. WASHINGTON: Magazine Article [Details]
2013 Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammation and angiogenesis in rheumatoid arthritis.
Connolly, MC;Rooney, PR;Maratha, AX;Miggin, SM;Veale, DJ;Fearon, UM (2013) Acute serum amyloid A is an endogenous TLR2 ligand that mediates inflammation and angiogenesis in rheumatoid arthritis. LONDON: Magazine Article [Details]
2013 Association Between Type 2 Diabetes & Inflammation.
Gupta, S;Maratha, A;Natarajan, A;Gajanayake, V;Siednienko, J;Hoashi, S;Miggin, S (2013) Association Between Type 2 Diabetes & Inflammation. LONDON: Magazine Article [Details]

Reviews

  Year Publication
2012 Cancer and viruses: A double-edged sword.
Butt, AQ;Miggin, SM (2012) Cancer and viruses: A double-edged sword. HOBOKEN: Reviews [DOI] [Details]